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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Profiling Differential Effects of 5 Selective Serotonin Reuptake Inhibitors on TLRs-Dependent and -Independent IL-6 Production in Immune Cells Identifies Fluoxetine as Preferred Anti-Inflammatory Drug Candidate
doi: 10.3389/fphar.2022.874375
Figure Lengend Snippet: Effects of SSRIs on IL-6 production induced by TLR4 or TLR9 agonist in J774.1 murine macrophage cells. J774.1 cells were pre-incubated with each SSRI (1–10 µM) for 30 min and then incubated with LPS (1 μg/ml) for 6 h (A) or 24 h (B) , or with CpG ODN or negative control ODN (200 ng/ml) for 6 h (C) or 24 h (D) . IL-6 was measured by means of ELISA. Error bars indicate ±SE [ (A) : n = 16, (B–D) : n = 12, three or more independent experiments]. Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. Significant differences between control and test groups are indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. NC, negative control ODN. PAX, paroxetine; FLX, fluoxetine; FVX, fluvoxamine; SER, sertraline; ESC, escitalopram.
Article Snippet:
Techniques: Incubation, Negative Control, Enzyme-linked Immunosorbent Assay, Control